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X-WR-CALDESC:Events for ISyN
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DTSTART:20220101T000000
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BEGIN:VEVENT
DTSTART;TZID=UTC:20230719T140000
DTEND;TZID=UTC:20230719T190000
DTSTAMP:20260501T054737
CREATED:20230618T093724Z
LAST-MODIFIED:20230717T124306Z
UID:1330-1689775200-1689793200@isyn-mainz.de
SUMMARY:Systems Club III - Light Sheet Microscopy
DESCRIPTION:Deeper Insights: Exploring LSM in Combination with Tissue Clearing \n\n\n\n\n\nLight Sheet Microscopy (LSM) allows revolutionizing high-speed\, high-resolution imaging and reduced photo-toxicity\, proving invaluable in whole-organ and whole-organism imaging. The combined power of LSM with tissue clearing now offers unparalleled views of intact organ structures\, fostering comprehensive investigations and rich insights into the cellular landscape.The Systems Club is delighted to host an event centering on this remarkable synergy. We will explore the principles of LSM\, focusing on its feature: the illumination of thin sections of samples. A spotlight will be on tissue clearing\, a process that removes light-scattering elements from tissues\, enabling the light to penetrate deeper and offer high-throughput imaging of intact organs or organisms. \n\n\n\nThe hands-on demonstration will guide you through a typical LSM setup\, highlighting the use of tissue clearing in this context. As part of the session\, the latest software tools\, ClearMap and CellFinder\, which facilitate an unbiased\, high-throughput analysis of neuronal activity in the whole mouse brain\, will be presented. To aid those with limited programming experience\, a user-friendly graphical interface to streamline these analysis tools was developed and will be also introduced.Join us on this journey\, as we untangle the complexities of LSM and tissue clearing\, and open up new possibilities for in-depth hollistic exploration. \n\n\n\n\n\n\n\n  2 – 3:30PM: Welcome\, Presentations and Discussion Round on:    \n\n\n\n\nTissue Clearing and Light Sheet Microscopy: Implications in Neuroscience\n\n\n\nComputational Analysis of Light Sheet Microscopy data\n\n\n\n\n 3:30 – 4:30PM: Lab Tour  \n\n\n\n\nLight Sheet Microscopy and Tissue Clearing\n\n\n\n\nWhile you are waiting for your next tour slot or if you are already done\, you can start joining the catering and open discussion . \n\n\n\n 4:30PM – Open End: Discussion with Catering and Wine   \n\n\n\n \n\n\n\n Registration:  \n\n\n\nRegistration is available after login – if you do not have an ISyN-Account yet\, create one\, using the “Become a member”-Button in the top. \n\n\n\nAfter you registered for this event\, you will get an e-mail asking you for some more details regarding e.g. tours you’d like to participate in.
URL:https://isyn-mainz.de/event/systems-club-iii-light-sheet-microscopy/
LOCATION:Biozentrum 1\, Hanns-Dieter-Hüsch-Weg 15\, Mainz\, RLP\, 55128\, Germany
CATEGORIES:ISyN-Event,Systems Club
ORGANIZER;CN="Lena Fr%C3%B6hlich":MAILTO:lena.froehlich@lir-mainz.de
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BEGIN:VEVENT
DTSTART;TZID=UTC:20220729T140000
DTEND;TZID=UTC:20220729T190000
DTSTAMP:20260501T054737
CREATED:20220307T101057Z
LAST-MODIFIED:20220629T135316Z
UID:1149-1659103200-1659121200@isyn-mainz.de
SUMMARY:Systems Club II - Mouse Behavioral Tracking
DESCRIPTION:Methods for deriving Resilience against stress in mice from long term observations \n\n\n\n\n\n \n\n\n\nWhen individuals in major psychological or physical stressful situations are able to maintain their mental health or restore it quickly after a short period of experiencing stress-related symptoms\, this is referred to as resilience. The classification of mice into resilient and susceptible animals based on tests such as chronic social defeat test\, is a common approach in resilience research. However\, this classification based on short-term observation has recently been viewed increasingly critically and its transferability to the definition of resilience in humans has at least been questioned. But which methods can give us information about the long-term behavior of mice and is this really helpful for determining the resilience status of a mouse? \n\n\n\n \n\n\n\nIn our Systems Clubs we want to encourage you\, to join the discussion. Bring your own opinions and if you think it is helpful\, feel free to bring a short presentation giving a new impulse on the topic at hand. This Systems Club is particularly aimed to discover different methods for mouse behavioral tracking and to discuss them together as well as how to define resilience in general.  \n\n\n\n\n\n\n\n  2 – 3:30PM: Welcome\, Presentations and Discussion Round on:    \n\n\n\n \n\n\n\nThe IntelliCage System and Data Analysis using IntelliPy (Nicolas Ruffini)VAME\, Deeplabcut & Analysing generic tracking and behavioral data using Resilipy (Vincent Dietrich)Using and analyzing data with the LiveMouseTracker System (Saskia Göbler & Nicolas Ruffini)\n\n\n\n \n\n\n\n 3:30 – 4:30PM: Short tours showing the Live Mouse Tracker System and/or the IntelliCage System \n\n\n\n \n\n\n\nTour on the IntelliCage SystemTour on the Live Mouse Tracker System\n\n\n\n \n\n\n\n While you are waiting for your next tour slot or if you are already done\, you can start joining the catering and open discussion . \n\n\n\n 4:30PM – Open End: Discussion with Catering and Wine   \n\n\n\n \n\n\n\n\n\n Registration:  \n\n\n\n Registration is available after login – if you do not have an ISyN-Account yet\, create one\, using the “Become a member”-Button in the top. \n\n\n\n After you registered for this event\, you will get an e-mail asking you for some more details regarding e.g. tours you’d like to participate in.
URL:https://isyn-mainz.de/event/systems-club-ii-mouse-behavioral-tracking/
LOCATION:Biozentrum 1\, Hanns-Dieter-Hüsch-Weg 15\, Mainz\, RLP\, 55128\, Germany
CATEGORIES:ISyN-Event,Systems Club
ORGANIZER;CN="Nicolas Ruffini":MAILTO:nicolas.ruffini@lir-mainz.de
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=UTC:20220429T140000
DTEND;TZID=UTC:20220429T190000
DTSTAMP:20260501T054737
CREATED:20220307T101055Z
LAST-MODIFIED:20220405T075904Z
UID:1132-1651240800-1651258800@isyn-mainz.de
SUMMARY:Systems Club I - Calcium Imaging Pipeline:
DESCRIPTION:From Data Acquisition to Analysis \n\n\n\n\n\nThe field of optical calcium imaging\, particularly of individual neuron function in the intact tissue\, relies on detecting subtle changes in light intensity. The implementation of 2-photon microscopy in combination with calcium indicators allowed for the first time a functional readout of neuronal circuits comprising of several hundred neurons in the rodent cortex. This led to a tremendous advance in our understanding on how complex circuit dysfunction arise\, particularly also in early stages of neurological disorders. Particularly 2 photon calcium imaging approaches pose unique challenges\, both in terms of system integration\, experimental design\, and not the least\, analysis. Most of these steps are processed quite differently depending on the laboratory. Here\, in a hands-on-manner\, we aim to show you our procedure of data acquisition and analysis\, including the GUI ViNe-Seg\, which has been recently developed in the Stroh-Lab. \n\n\n\nIn our Systems Clubs we want to encourage you to join the discussion. Bring your own opinions and if you think it is helpful\, feel free to bring a short presentation giving a new impulse on the topic at hand. This Systems Club is particularly aiming for initiating a discussion of different procedures for image processing and downstream analyses of calcium imaging data. \n\n\n\n\n\n\n\n The planned time table is the following:  \n\n\n\n 2 – 3:30PM: Welcome\, Presentations and Discussion Round on:   \n\n\n\n \n\n\n\nThe Data Acquisition (Hendrik Backhaus)The Autosegmentation\, Trace Extraction\, Baseline Estimation and Peak Detection (Nicolas Ruffini\, Saleh Altahini\, Hendrik Backhaus)The Downstream Analysis (Anna Wierczeiko\, Nicolas Ruffini)\n\n\n\n \n\n\n\n 3:30 – 4:30PM: Short tours showing the Experimental Calcium Imaging Setup in RG Stroh  \n\n\n\n \n\n\n\n2-Photon Microscope TourMiniscope & Electrophysiology Tour\n\n\n\n \n\n\n\nWhile you are waiting for your next tour slot or if you are already done\, you can start joining the catering and open discussion. \n\n\n\n 4:30PM – Open End: Discussion with Catering and Wine  \n\n\n\n \n\n\n\nRegistration: \n\n\n\nRegistration is available after login – if you do not have an ISyN-Account yet\, create one\, using the “Become a member”-Button in the top \n\n\n\nAfter you registered for this event\, you will get an e-mail asking you for some more details regarding e.g. tours you’d like to participate in.
URL:https://isyn-mainz.de/event/systems-club-i-calcium-imaging-pipeline/
LOCATION:Biozentrum 1\, Hanns-Dieter-Hüsch-Weg 15\, Mainz\, RLP\, 55128\, Germany
CATEGORIES:ISyN-Event,Systems Club
ORGANIZER;CN="Nicolas Ruffini":MAILTO:nicolas.ruffini@lir-mainz.de
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