At ISyN, we aim for new concepts of method training and interaction of neuroscientists at all levels of education.
We invite the community, particularly PhD students and PostDocs, to actively participate and shape the SystemsClub.
The SystemsClub focuses on the exchange and training of the scientists carrying out the respective scientific projects. In many cases, system-theoretical and data-driven methods are generic and can be applied to different questions by incorporating the relevant expert knowledge from the various areas of neuroscientific research.
Each SystemsClub event addresses a specific subject, being hosted by a specific research group. One or more talks on this topic will be given by the respective host and by speakers from other research groups. The talks should focus on the current problems and roadblocks in terms of Systems Analyses. These could be manifold, such as problem of small data, whether to implement machine learning, which programming language to use, how to extract the features of interest from noisy data, data integration in regards to different level observations. Note, you do not need to be an analysis expert to host the SystemsClub! The talk(s) will be followed by an extended open-end discussion. Often times, a lab tour and a subsequent get together will be quite useful.
The field of optical calcium imaging, particularly of individual neuron function in the intact tissue, relies on detecting subtle changes in light intensity. The implementation of 2-photon microscopy in combination with calcium indicators allowed for the first time a functional readout of neuronal circuits comprising of several hundred neurons in the rodent cortex. This led to a tremendous advance in our understanding on how complex circuit dysfunction arise, particularly also in early stages of neurological disorders. Particularly 2 photon calcium imaging approaches pose unique challenges, both in terms of system integration, experimental design, and not the least, analysis. Most of these steps are processed quite differently depending on the laboratory. Here, in a hands-on-manner, we aim to show you our procedure of data acquisition and analysis, including the GUI ViNe-Seg.
The field of studying resilience against stress in mice through long-term observations involves various methodologies aimed at understanding the mechanisms underlying an organism’s ability to adapt and recover from stressful situations. This research primarily relies on observing and analyzing behavioral and physiological changes in mice over extended periods of time. By monitoring responses to stressors, researchers can gain valuable insights into the factors contributing to resilience and potential therapeutic interventions. Here, we aim to show you different methods for deriving resilience in mice from long term observations, including the IntelliCage System and the LiveMouseTracker System.
Light Sheet Microscopy (LSM) allows revolutionizing high-speed, high-resolution imaging and reduced photo-toxicity, proving invaluable in whole-organ and whole-organism imaging. The combined power of LSM with tissue clearing now offers unparalleled views of intact organ structures, fostering comprehensive investigations and rich insights into the cellular landscape. The hands-on demonstration will guide you through a typical LSM setup, highlighting the use of tissue clearing in this context. As part of the session, the latest software tools, ClearMap and CellFinder, which facilitate an unbiased, high-throughput analysis of neuronal activity in the whole mouse brain, will be presented. To aid those with limited programming experience, a user-friendly graphical interface to streamline these analysis tools was developed and will be also introduced.